Located in Nashville, Tennessee, and operating at a global crossroads of teaching, discovery and patient care, VUMC is a community of individuals who come to work each day with the simple aim of changing the world. It is a place where your expertise will be valued, your knowledge expanded and your abilities challenged. It is a place where your diversity — of culture, thinking, learning and leading — is sought and celebrated. It is a place where employees know they are part of something that is bigger than themselves, take exceptional pride in their work and never settle for what was good enough yesterday. Vanderbilt's mission is to advance health and wellness through preeminent programs in patient care, education, and research.
This laboratory is focused on understanding processes that promote inflammation and vascular growth in the retina with the ultimate goal of finding new treatments for diabetic retinopathy and retinopathy of prematurity. Due to the growing incidence of obesity and diabetes in the pediatric population, and to advances in neonatal care allowing younger preemies to survive, the prevalence of these conditions is expected to increase.
In diabetic retinopathy and retinopathy of prematurity, inflammation and new vessel growth in the retina can lead to vision loss from swelling, bleeding, scarring, and tissue traction. Our goal is to repurpose drugs previously developed to target inflammation and vascular growth in non-ocular systems to prevent, abort, or revert them in these retinopathies. We use human retinal cells from donor eyes as well as animal models to achieve these goals. Both pharmacologic and genetic approaches are employed in our lab to block the function of target receptors (inhibitors, siRNA, or neutralizing antibodies in culture, and receptor knockout mouse strains or pharmacologic inhibition by drug delivery in animal models of disease) in order to discover and/or develop new therapies.
We are looking forward to working with a highly motivated individual who can collaborate with others in lab and who exhibits a high level of professionalism, dedication, reliability, intellectual curiosity and technical expertise.
Varied Shift ; Weekend or after-hours work may be required, as needed
Under minimal supervision, set up, conduct experiments, and continue research projects following protocols including the appropriate recording of data and reporting of standard or variant results. Assist in other laboratory and research functions.
Key Functions and Expected Performances:
Under minimal supervision, plan and conduct complex experiments, analyze data, prepare reports, and archive data.
Plan and coordinate experiments in conjunction with Principal Investigator (PI).
b. Process and analyze data demonstrating keen analytical and problem solving skills.
Understand and follow experimental protocols and competently perform any required assays (may include animal handling, tissue culture, etc.)
Accurately record/document, compile, organize, interpret, and communicate data/results.
Follow University safety protocols (may include use of toxic and hazardous materials, and/or carcinogens).
Perform calculations involving molarity, % solutions, and serial dilutions.
g. Perform data analysis and create graphs
Operate and maintain equipment, and, troubleshoot equipment malfunctions throughout the execution of all research projects associated with the lab.
Recommend equipment and supplies needed for research projects.
Read and follow all equipment manuals.
Set up, maintain, troubleshoot and arrange to repair equipment according to manufacturer and Vanderbilt University guidelines.
Ensure laboratory is prepared for experiments.
a. Procure, organize and prepare lab supplies needed to complete the work.
Maintain sterile conditions as required.
Assist PI in modifying procedures and techniques.
Recognize problems and suggest solutions, assist in modifications.
Recommend improvements to established techniques and/or protocols (troubleshooting).
Maintain personal knowledge of scientific trends through literature reviews.
Provide appropriate written/oral documentation of experiments.
a.Effectively communicate in oral and written formats.
Prepare research progress reports.
Analyze and summarize test results.
Assist in the effective operation of the laboratory.
Train other employees.
Supervise projects and/or personnel.
Serve as a liaison to other Vanderbilt Medical School Departments, Divisions and core facilities such as: Division of Animal Care (DAC), Institutional Animal Care and Use Committee (IACUC), Vanderbilt Environmental Health and Safety (VEHS) etc, to ensure compliance of laboratory practices and procedures with University, local and federal rules and regulations.
Preferred Education, Skills, and Experiences:
Bachelor's degree in Molecular Biology, Biochemistry, or related field highly desired
2-3 years of rigorous laboratory experience or the equivalent
Desired Qualifications :
MS/PhD with at least 3 years of previous lab experience
Experience in an academic research lab
Preference will be given to candidates with a demonstrated ability to communicate research findings through publication in high impact, peer-reviewed journals and presentations at national meetings.
Preference will be given to individuals who want to establish themselves in a stable long employment relationship with the laboratory and who are interested in continued professional development.
Laboratory skills that are sought include:
o Breeding mice and working with animal models of human disease.
o Histology: sectioning, immunohistochemistry, imaging.
o Protein purification and analysis: WB, ELISA, IPs.
o Cellular and molecular biology: transfections, qRT-PCR, genotyping.
o Cell/Tissue cultures: cell cultures and in-vitro cell assays.
o Toxicity/pharmacokinetic assays using inhibitors.
Examples of lab Assays:
Cell culture (human retinal endothelial cells, human Muller cells, human microglia)
Cell migration assays
Cell proliferation assays
Tube formation assays
Cytokine induction assays (ELISA, RT-PCR)
Receptor activation assays (WB)
Proteome profiling assays
Trans-endothelial electrical resistance assays
Leukocyte adhesion assays
Mouse colony management (breeding and genotyping mice)
Live retinal imaging and functional assays (optical coherence tomography, fluorescein angiography, electroretinogram (ERG))
Retinal dissection and ex-vivo analysis after therapy (leukostasis assays, vascular leakage assay (Evans blue))
VUMC Recent Accomplishments
Because we are committed to providing the best in patient care, education and research, we are proud of our recent accomplishments:
· US News & World Report: #1 Hospital in Tennessee, #1 Health Care Provider in Nashville, #1 Audiology ( Bill Wilkerson Center ), 12 adult and 10 pediatric clinical specialties ranked among the nation's best, #15 Education and Training
· Truven Health Analytics: among the top 50 cardiovascular hospitals in the U.S.
· Becker's Hospital Review: one of the "100 Greatest Hospitals in America"
· The Leapfrog Group: grade "A" in Hospital Safety Score
· National Institutes of Health: among the top 10 grant awardees for medical research in the US
· Magnet Recognition Program: Vanderbilt nurses are the only group honored in Tennessee
· Nashville Business Journal: Middle Tennessee's healthiest employer
· American Hospital Association: among the 100 "Most Wired" medical systems in the US
Vanderbilt University Medical Center is home to Vanderbilt University Hospital, The Monroe Carell Jr. Children’s Hospital at Vanderbilt, the Vanderbilt Psychiatric Hospital and the Vanderbilt Stallworth Rehabilitation Hospital. These hospitals experienced more than 61,000 inpatient admissions during fiscal year 2015. Vanderbilt’s adult and pediatric clinics treated nearly 2 million patients during this same period.Vanderbilt University Hospital and the Monroe Carell Jr. Children’s Hospital at Vanderbilt are recognized again this year by U.S. News & World Report’s Best Hospitals as among the nation’s best with 18 nationally ranked specialties. Vanderbilt University Medical Center is world renowned because of the innovation, work ethic and collegiality of its employees. From our health care advances to our compassionate care, Vanderbilt owes its accomplishments and reputation to staff and faculty who bring skill and drive and innovation to the medical center day after day. World-leading academic departments and comprehensive centers of excellence pursue scientific discoveries and transformational educational and clinical advances across the entire spectrum of health and disease.As th...e largest employer in middle Tennessee, we welcome those who are interested in ongoing development in a caring, culturally sensitive and professional atmosphere. Most of us spend so much of our lives at work, we want to be part of maintaining a workplace in which people support one another and encourage reaching for excellence. Many high-achieving employees stay at Vanderbilt because of the professional growth they experience and because of their appreciation of Vanderbilt’s benefits, public events and discussions, athletic opportunities, beautiful setting and, above all, sense of community and purpose.Vanderbilt and its employees share a set of mutual expectations that have been created with productivity, legality, fairness and safety always in mind. We believe that our investment in training and compensating employees multiplies in value when we enable individuals to deliver their best performance for the benefit of us all.